Week 10 of the quarter! Two more weeks of academic instruction before free-for-all time begins! It feels incorrect that we are here and yet here were are!!!!! Time to lay down like Syril.

November Goals Recap

November Goals

Green Crab Pilot Experiment:

• FINISHED MY DRAFT MANUSCRIPT AND SENT IT TO CAROLYN!

Plan Biol 191:

• Finalized my syllabus!
• Sent a list of equipment to purchase for the course

West Coast Green Crab Experiment:

• Came up with an analysis plan for the RNA-Seq data
• Fought for my life in order to get trinity running
• Performed exploratory PCA and perMANOVA analyses, reviewed potential outliers, and performed exploratory beta dispersion tests
• Performed PLDAS for temperature and time and temperature and genotype
• Performed ASCAs for temperature and time and temperature and genotype

Other:

• Ordered molecular equipment!
• Networked with other green crab people in Elkhorn Slough

December Goals

West Coast Green Crab Experiment:

• Finish initial metabolomics analysis
• Complete transcriptome assembly
• Global gene expression differences by temperature, time, and genotype
• SICB talk

Plan Biol 191:

• Order physiology equipment
• Update Camino site
• Create rubrics for assignments
• Sign up for Camino accesbility course
• Course material for weeks 1-3

Other:

• Sort through -80ºC, -20ºC, and 4ºC
• Label and organize lab

Back Burner

I haven’t worked on these projects in a while, but they are next on my to-do list. I don’t think they need to be in the deep freeze so I guess I gotta turn the back burner on!

Hawaii Gigas Methylation:

• Revise methylKit code
• Run bismark with the new C. gigas genome
• Update methods and results
• Revise discussion
• Revise introduction
• Revise abstract

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Coral Transcriptomics:

These are things I need to do and can probably knock out in a week, but I think they’re a lower priority right now for me since there’s more momentum on other projects. At the very least, I need to reach out to collaborators and get this going again.

• Revise abstract
• Write discussion
• Write introduction