I’ve never felt more seen by a government website.

We’re somehow in Week 7 of the quarter! Some things are chugging along well, other things are……maybe not chugging. Let’s take some inventory.

October Goals Recap

October Goals

Green Crab Pilot Experiment:

• Used ASCA analysis for individual -omic datasets
• Realized I was missing some information in the methods and results, so I updated things
• Used Gemini as a launch pad for targeted literature search. It was a meh experience.
• I am SO CLOSE to having this discussion finished!

Biol 1C:

• We are chugging along (and thankfully we are no longer in the part that requires me to think a little too hard)

Plan Biol 191:

• Got my CDFW permit!
• Started purchasing molecular equipment!
• Priced out all physiology equipment!
• Sent Daryn list of class-specific equipment!
• Ordered the recirculating seawater system!
• Sorted through existing sample and falcon tubes!
• ……..my poor syllabus is very neglected.

West Coast Green Crab Experiment:

• Revised TTR and [respirometry](https://yaaminiv.github.io/Green-Crab-Experiment-2023-Part70/ data analyses
• Ignoring getting SCU cluster access for now since all my data is still on WHOI servers and it would be a pain to transfer things
• Started to QC RNA-Seq data. Forgot how long everything takes (especially when you are trying to remember how it works)
• Have not touched metabolomics data yet

Other:

• Finished IRB training modules for RIE2
• Got the RIE2 IRB approved!
• Finished the Generative AI course

November Goals

Green Crab Pilot Experiment:

• Finish discussion
• Write introduction
• Write abstract
• Send draft to Carolyn!

Plan Biol 191:

• Draft syllabus
• Get syllabus feedback and revise
• Finalize equipment and fieldwork asks for course

West Coast Green Crab Experiment:

• Finish preliminary RNA-Seq analysis
• Finish preliminary metabolomics analysis

Other:

• Sort through -80ºC, -20ºC, and 4ºC
• Label and organize lab
• Finish ordering molecular equipment
• Finish ordering physiology equipment
• Network with green crab and oyster people in Northern California

Back Burner

I haven’t worked on these projects in a while, but they are next on my to-do list. I don’t think they need to be in the deep freeze so I guess I gotta turn the back burner on!

Hawaii Gigas Methylation:

• Revise methylKit code
• Run bismark with the new C. gigas genome
• Update methods and results
• Revise discussion
• Revise introduction
• Revise abstract

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Coral Transcriptomics:

These are things I need to do and can probably knock out in a week, but I think they’re a lower priority right now for me since there’s more momentum on other projects. At the very least, I need to reach out to collaborators and get this going again.

• Revise abstract
• Write discussion
• Write introduction