Gigas Larvae Day 1
I’m the Mother of Oysters
When I first came in, I used PSRF’s temperature gun to check the temperature of each bucket (found here). I noticed that there was some temperature variation between totes, and that the totes in the middle seem to be holding temperature better than those on the edge. I changed the setpoints in all totes with more than one bucket (i.e. every tote except the far left one that I designated Tote 1) to 25 ºC s that the water temperature says around 23-24 ºC. I noticed there was some evaporation from the buckets, so I added a bit more water to each bucket.
I then fed the oysters C.iso with Ashley. I’m keeping track of my feeding here. After using the hemocytometer to count algae, I added half of the needed amount of algae to the buckets and letting it set for a bit. This way, I would give the larvae a chance to feed before saturating the tank some more. I also realized that I severely underfed my oysters on Sunday — they only got one-tenth of the food they should! Not sure why Rhonda and I messed up that calculation, but at this phase they don’t eat too much so I don’t think I’ll suffer a super large mortality.
Figure 1. Buckets after addition of half of algae quantity needed.
Since Joth was here today, I talked to him about some basic larval rearing things. He advised that I feed either C.iso or T.iso, 609 (Pavlova) and another diatom every day. We also talked about using epinephrine to induce setting as single oysters. He’s going to email me the protocol so I can order the epinephrine. This is something I’ll do when oysters are holding on a 180 micron screen. Joth also said I can use his tanks outside to set up a system of downwellers and upwellers for oyster post-set.