It’s the final countdown for SO. MANY. THINGS. this month! I need to be confronted with this ransom note-style threat every time I close my eyes.

March Goals Recap

Cold Acclimation Green Crab Experiment:

Found out midway through this month that my manuscript deadline was April 15, and not May 1! I spent most of my time this month working on this project.

• Finished genotyping WA crabs and revised TTR data based on updated WA genotypes!
• Genotyped Catlin’s crabs!
• Analyzed water temperature data for WA and Julia’s experiment
• Revised modeling for 2024 and Julia’s experiments
• Conducted population comparison for 2024 experiments
• Updated methods and results

West Coast Green Crab Experiment:

• Sorted through notes to understand which crabs were sampled and resolve inconsistencies
• Identified samples for metabolomics and transcriptomics sequencing
• Identified library prepration method and ordered samples
• Identified transcriptomics sequencing facility
• Reorganized -80ºC
• Cleaned the RNA room
• Started RNA extractions

Perspective Piece:

• Finished writing 3/4 of manuscript text
• Created plan for boxes and figures
• Identified key words for Web of Science and ProQuest searches

April Goals

Cold Acclimation Green Crab Experiment:

• Revise temperature data analysis
• Analyze Catlin’s TTR data with genotype
• Analyze Catlin’s temperature data
• Finalize methods and results
• Outline introduction
• Write introduction
• Outline discussion
• Write introduction
• Revise abstract
• Send to co-authors
• Submit to ICB!

West Coast Green Crab Experiment:

• Move RNA samples for extraction to the RNA room -20ºC
• Extract all RNA samples
• Find and consolidate all metabolomics samples
• Send metabolomics samples for seqeuencing
• Test library preparation kit
• Prepare sample libraries
• Send RNA libraries for sequencing

Perspective Piece:

• Finish writing
• Finish literature searches
• Make boxes and figures
• Send to collaborators for review
• Identify funds for open access publishing

Back Burner

I haven’t worked on these projects in a while, but they are next on my to-do list. I don’t think they need to be in the deep freeze so I guess I gotta turn the back burner on!

Green Crab Pilot Experiment:

• Finish -omics integration analysis
• Finish -omics and physiology integration analysis
• Update methods
• Update results
• Write discussion
• Write introduction
• Write abstract

Hawaii Gigas Methylation:

• Revise methylKit code
• Run bismark with the new C. gigas genome
• Update methods and results
• Revise discussion
• Revise introduction
• Revise abstract

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Coral Transcriptomics:

These are things I need to do and can probably knock out in a week, but I think they’re a lower priority right now for me since there’s more momentum on other projects. At the very least, I need to reach out to collaborators and get this going again.

• Revise abstract
• Write discussion
• Write introduction