Retracing my steps

Everything sucks.

R Help is Helpful

All aboard the NMDS Struggle Bus

…Everybody Everywhere

Clean up, clean up

September Goals

The end.

We have a Game Plan

The Final Countdown

They’re still not growing!

Checked the 80 micron screen again

Totes are pesky

Immersion heaters are pesky

Anoxia is no bueno

Smooth Saturday screening

Lots of maintenance today

My larvae are 60 microns now!

Counting larvae takes a while

A bit of a rough start today

I only have five goals

I’m the Mother of Oysters

Spoiler: We have baby oysters!

NO OYSTERS DIED!

It’s getting ugly

The mortalities continue

Revised for the last time

My prior calculations were incorrect!

Featuring reduced crosses and pools

Temperature is at 23 ºC!

If you don’t have a visualization, did you really analyze data?

My samples are DONE.

Last day of my samples!

Status quo?

Murphy’s Law is always relevant

The power is back!

Still plugging along

Power outage edition

More borrowed PRTC = All samples prepared

Our assay works!

It’s SRM assay time!

WE HAVE OUR TARGETS.

Those peaks are running interference

And the pH is low (again)

I have draft transitions!

Differential Expression + Protein Function = Targets?

The pH is kind of stable?

MSstats works!

June was rather slow

It’s time to think about crosses!

Revised (final?) Conditioning Plan

Sophie’s New Choice: Temperature, Flow or pH?

It’s been a slow burn

I was gifted 3,000 extra proteins

This time it only took 4 hours!

Remember that Skyline fail?

From Saturday 6/10 to Wednesday 7/5

Last day of DNR extractions (hopefully)!

Time to fatten the oysters up!

It’s getting hot in here

Yaamini vs. CentriVap: The Sequel

(Almost) done with Spring Quarter already?!

I am now a faster pipetter

All this protein but I’m still not swole.

Here’s hoping this is the last round of DNR sample preparation!

I need to become a faster pipetter.

We have lots of protein!

How many times can you restart sample preparation?

Slice and dice

More cowbell? More samples.

The DIA pipeline has been quite a #strugglebus

It’s always good to practice your methods of torture.

Who wants to manually quality check peptides?!

Record scratch. Freeze frame.

Who are the lucky winners?

It’s time to extract more protein.

I can now analyze my full .blib!

It’s already MAY.

Skyline works with our demultiplexed files!

It’s not as easy as clicking a button

Laura went out to Manchester today and graciously cleaned my oysters

I became well-aquainted NCBI SRA database today

I’m going to get stuff done for Pubathon (I promise)!

My files are done converting!

Another new MSConvert CLI

New MSConvert CLI, hot off the presses

Demultiplexing is perplexing

OA System Breakdown

We’re done with our OA experiment!

I was so busy catching up on classes I forgot it was April!

What happened when Laura got stranded in the Midwest

I’m at NSA, to Grace and Olivia went to Manchester.

Here’s the evolution of my poster for NSA!

Checking in before we leave for NSA

On that number-crunching grind

We have data!!!

A couple hiccups at Manchester today

It works!

Yesterday I set up a system for cataloging our water chemistry data.

Reformatting PECAN inputs

New day, same errors

We’re officially halfway through our pH treatment window!

To reconvert or not reconvert?

Hit an obstacle with PECAN this weekend.

Another smooth day at Manchester!

:cake: It’s not as easy as pie :cake:

March Maddness is upon us.

Today, I spent 3 hours cleaning oyster :poop:

The OA System is up and running!

February Goals Update

Conducted my first outreach activity today!

First Manchester Sampling

February Goals

…But now trouble is gone!

…And trouble stuck around at 3:51 p.m. on January 29.

Trouble struck at 8:16 p.m. on January 28.

My samples are on the Mass Spec!

Mass Spectrometer Set-up

M/Z ratios: Mass hysteria ensues

PRTC: We know what those peptides can be

Not Salty about Desalting

Speed Vacuum = Slow Process

Trippin for Trypsin

If at first you don’t succeed, trial trial again.

Practice makes perfect?

Gill Tissue + Sound Waves = Protein Soup

My first day in lab was a busy one!

My DNR project is still on the brain!.

Now that it’s almost November, it’s time to get cracking on the DNR project.

As promised, here is some background on my current project

Hello!