July Goals Recap:

Coral Methylation Comparison:

• Updated the methods and results for the revised statistical analyses
• Added to the introduction and discussion

Gigas and Virginica Labwork:

• Revised health and safety plan
• Created a priority list for samples
• Checked quantities of MethylMiner kit reagents and ordered more kits
• Started shearing sperm DNA samples for MBD enrichment and checked quality
• Selected C. gigas gonad DNA samples for WGBS and checked concentrations

Side Projects:

• Conducted a WGCNA and GO-MWU analysis for Z. marina transcriptomics
• Created figures and revised text for ocean acidification and reproduction review

August Goals

Coral Methylation Comparison:

• Update manuscript as needed

Virginica Sperm Labwork:

• Finish shearing and quality checking samples
• Perform MBD enrichment
• Prepare libraries and send for sequencing

Gigas Gonad Labwork:

• Finish checking DNA quality
• Conduct isopropanol precipitations for DNA
• Send DNA for sequencing
• Start RNA isolations

Other:

• Finish Z. marina analyses and ocean acidification and reproduction review
• Review Ecological Applications manuscript
• Create a timeline for postdoc funding applications