Full Skyline Preliminary Analysis

I was gifted 3,000 extra proteins

When error checking the new Skyline document from the revised “desearlinated” .blib, I noticed that I had 9,047 proteins instead of about 6,000. Where did these 3,000 extra proteins come from? What do they do? To answer this question, I ran the new Skyline output through the same preliminary pipeline I used for my NSA poster.

Jupyter notebook

Videos of my narrating my findings quietly because I’m at my parents’ house and they’re asleep:

Part 1

Part 2

Most interesting finding: miRNA inhibition of translation is a significant GO term for biological processes that’s overrepresented across all of my samples!


Written on June 13, 2017