Treatment Days 44-49

THE END.

8/11/23:

• No mortalities!
  • T1: 6
  • T4: 8
  • T7: 6
• Fed all tanks 1/8 tsp of food

8/12/23:

• No mortalities!
  • T1: 6
  • T4: 8
  • T7: 6
• Ammonia check and conditioner added when ammonia > 1.0. 60% of water replaced in all tanks
  • T1: 0.5
  • T4: 2.0
  • T7: 1.0
• Changed filters and fed all tanks 1/4 tsp of food so I wouldn’t have to come in tomorrow

8/14/23:

• No mortalities!
  • T1: 6
  • T4: 8
  • T7: 6
• Fed all tanks 1/4 tsp of food so I wouldn’t have to come in tomorrow
• Labelled tubes. Unknown status (not sure if alive or dead based on spreadsheet for the following crabs, seem like anomalies based on current survivorship)
  • T4: 5-066, 5-075
  • T7: 5-134

8/16/23:

• T1
  • Salinity: 39
  • 5-015: Guessed the crab number based on the final digit of the label, which was correct.
• T4
  • Salinity: 39
  • 5-066: NOT THERE. This makes sense! When I combed through the TTR spreadsheets and my old notes, I realized that 5-066 was sampled 7/5/23 and placed in a tube that said 5-060.
  • 5-068: Escaped after we did TTR! We found it hiding behind the dry ice cooler at the end of the experiment when we were cleaning up. Since it hung out for a while in air, sampling it for the experiment didn’t make any sense.
  • 5-075: Sampled normally :)
  • Probe/chamber 2: switched during background because the fitting was coming off
• T7:
  • Salinity: 39
  • 5-134 —> NOT THERE. Makes sense because it died 7/23/23!

THE EXPERIMENT IS FINALLY DONE! I’m sure other things will come up as I clean data but for now it’s time to sleep.

Going forward

1. Assist Lauren and Julia with final presentations
2. Test formatting and analysis for one set of respirometry data
3. Respirometry analysis
4. Update methods
5. Update results