A quick recap of things I’ve revised

Just so there’s a record somewhere, here’s what I’ve done!

Posthoc tests and modifying figures

Carolyn suggested I look into posthoc tests! It was a lot more than I had capacity to figure out so I sadly needed to use ChatGPT. The transcript can be found here. A quick summary of things that I needed to do:

• Modify timepoint to be categorial for Catlin and Julia’s studies since they are proxies for temperature treatments
• Run posthoc tests with emmeans
• Lost my mind but managed to add text and asterisk labels highlighting significant differences
• Not related, but I modified the orientation of some plots! I also added some additional annotations to the temperature plots.

Figures 1-6. Modified plots

SST + map plot

Carolyn pulled down SST data for me and roughly plotted the data. I took that code, modified it, and figured out how to make a nice map with ggplot in this script! I was very annoyed that I couldn’t get the two plots to be the same size when I was sticking them together with patchwork and cowplot. But whatever. It’s done.

Figure 7. Study site map and SST

Supplementary material

I also tackled the supplementary material, particularly Julia’s respirometry analysis. She sent me her code which I modified. While she imported data for both timepoints, she fully processed the data only for the final timepoint! I decided to forge ahead with the only final timepoint because I think it would answer the main question of how priming alters physiological responses.

Figures 8-9. Supplementary figure

Going forward

1. Finalize manuscript
2. Submit the paper!
3. Troubleshoot lipid assay protocol
4. Conduct lipid assay for crabs of interest