This, but experimental logistics and data analysis when you’ve also taken 2.5 weeks off. It was really brave of me to think that I’d get any heavy work done last month!

May Goals recap

Green Crab Summer Experiment:

• Came up with project ideas for Julia and Lauren!
• Ordered almost all needed experimental materials

Green Crab Pilot Experiment:

• Completed the demographic data analysis
• Updated methods and results
• Didn’t get a chance to start the respirometry analysis since I shifted focus to the 2023 experiment logistics

Other:

• Started the Mindful Productivity course!

June Goals

Green Crab Pilot Experiment:

• ANALYZE. THE. RESPIROMETRY. DATA. Preferrably before I start this summer’s experiment
• Send metabolomics samples to UC Davis for sequencing

West Coast Green Crab Experiment:

• Finish buying all experimental materials
• Set up ESL
• Receive the Washington crabs!!
• Label and measure all crabs for the experiment
• Start the temperature treatments!!

Summer Students:

• Julia
  • Finalize summer project
  • Write letter for senior thesis proposal
  • Review SSF Research and Communication Plan
  • Review senior thesis proposal
• Lauren
  • Finalize summer project
  • Complete mentor-mentee agreement
  • Order any necessary materials
  • Identify test samples for summer assays

Hawaii Gigas Methylation:

• Add Rajan’s edits as comments to the draft manuscript
• Review EpiDiverse output and create a new SNP list
• Rerun methylKit
• Conduct a randomization test for DML identification
• Update methods and results

Virginica Methylation and Expression:

• Update methods and results from quadratic addition to transcriptional noise model
• Compare transcriptional noise analysis with Liew and Aranda papers

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Killifish Methylation and Expression:

I’ll return to this when I have a good draft of the coral paper.

• Pathway analysis for RNA-Seq data
• Determine protocol for SNP identification
• Conduct in silico RRBS digestion
• Characterize DEG that overlap between populations
• Quantify gene expression variability
• Visualize DEG data

Zebrafish DNMT:

I’ll think about this once I have a robust killifish paper draft.

• Identify a stress condition to use for the zebrafish based on disease factors or conditions that are known to induce changes in methylation
• Determine physiological endpoints to assess for embryos
• Construct a project timeline