YIKES I haven’t made a goals post yet this year! January was a blur with SICB, vacation, and a job interview. February was devoted to labwork. So let’s see where things stand? And of course, outline the many overlapping goals I have for this month.

December Goals Recap

Cold Acclimation Green Crab Experiment:

• Ran into several hiccups, but FINALLY finished genotyping MA crabs in February!
• Genotyped WA crabs that were alive for the whole experiment in December! Made good progress on the remaining crabs in February.
• Incorporated genotype into preliminary MA TTR analysis
• Incorporated genotype into preliminary WA TTR analysis

Green Crab Pilot Experiment:

I HAVEN’T DONE ANYTHING FOR THIS AND IT MAKES ME SAD.

NSF Grant:

• Finalized project summary!
• Shared the project summary with a program officer at SICB
• Then all hell broke loose with the new administration so this grant writing is being tabled indefinitely

West Coast Green Crab Experiment:

• Budgeted remaining NSF money
• Started getting quotes for metabolomics and transcriptomics sequencing

Other:

• Presented a talk on the 2024 data at SICB!
• Started on the ICB perspective paper
• Started on the ICB genotype and acute stress paper
• Re-familiarized myself with the Hawaii paper for an April aquaculture special issue
• GOT A JOB OFFER AND SUCCESSFULLY NEGOTIATED AND SIGNED!! More on that in a future lab notebook post

March Goals

Green Crab Pilot Experiment:

• Finish -omics integration analysis
• Finish -omics and physiology integration analysis
• Update methods
• Update results
• Write discussion
• Write introduction
• Write abstract

Cold Acclimation Green Crab Experiment:

• Finish genotyping WA crabs
• Genotype Catlin’s crabs
• Write methods
• Write results
• Write discussion

West Coast Green Crab Experiment:

• Identify samples for metabolomics and transcriptomics sequencing
• Send metabolomics samples out for sequencing
• Identify transcriptomics sequencing facility
• Pay for transcriptomics services before March 25
• Clean the RNA room
• Start RNA extractions

Perspective Piece:

• Finish manuscript text
• Draft boxes and figures

Hawaii Gigas Methylation:

• Revise methylKit code
• Run bismark with the new C. gigas genome
• Update methods and results
• Revise discussion
• Revise introduction
• Revise abstract

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Coral Transcriptomics:

These are things I need to do and can probably knock out in a week, but I think they’re a lower priority right now for me since there’s more momentum on other projects. At the very least, I need to reach out to collaborators and get this going again.

• Revise abstract
• Write discussion
• Write introduction