#humpdaymotivation

April Goals Recap:

i.e. me marveling at the ambition I had

Virginica Gonad Methylation:

• Modified paper repo so analysis is easily reproducible
• Refined my DML analysis and (almost) finished refning the general methylation analysis
• Didnt’ get to DMR or gene-level analysis because I am still sorting out issues with the general methylation trend analysis

Virginica Sperm:

• I was focusing on the gonad methylation paper so I haven’t thought about lab work

Other:

• Gave a talk at the SAFS Centennial Celebration and public lecture at Town Hall Seattle!
• Between the current paper and giving two talks, my milestone progress took a back seat

May Goals

i.e. me trying to set some realistic expectations for this month while also trying to achieve everything I didn’t do last week and making progress beyond that

Virginica Gonad Methylation:

• Troubleshoot CpG counts and finalize general methylation trends by May 3
• Preliminary description of genes with differentially methylated loci by May 5
• Differentially methylated region analysis by May 7
• Differentially methylated gene analysis by May 9
• Discussion outline by May 9
• Draft paper by May 12
• Paper submission to bioRXiv by May 31

Gigas Gonad and Larvae Methylation:

• Have MBD workflow meeting
• Extract RNA
• Process DNA for bisulfite sequencing
• Send DNA and RNA for sequencing
• Develop plan for processing larval samples

Virginica Sperm Methylation:

• Identify samples for RNA-Seq
• Extract RNA
• Process DNA for bisulfite sequencing
• Send DNA and RNA for sequencing

Other:

• Identify a GSR
• Schedule a committee meeting for summer