I had a very ambitious plan for September! Instead, I kinda treaded water and recharged while on vacation. Much like Moo Deng, my motivation was not present for anything that involved work. Perhaps there’s now a fire lit under me that will keep me going?

September Goals Recap

Green Crab Pilot Experiment:

• Finalized lipidomics analysis and WCNA!
• Finalized metabolomics enrichment analysis!
• Finalized lipidomics enrichment analysis!
• Dug into enrichment results!

West Coast Green Crab Experiment:

• Revised analytical methods for TTR and respirometry!

Killifish Methylation and Expression:

• Updated methods and results

Job Applications:

• Created job application schedule
• Updated job application materials
• Applied for relevant jobs

Other:

• Worked on invited seminars for Bigelow and Colby College
• CEABiGR paper was accepted for publication!

October Goals

Green Crab Pilot Experiment:

• Repeated measures analysis without crab ID for TTR data
• Determine if physiology data can be integrated with -omics data
• Determine if metabolomics and lipidomics data can be integrated
• Update methods
• Update results
• Write discussion
• Write introduction
• Write abstract

NSF ORCC:

• Reread relevant literature
• Finalize project summary
• Full grant draft
• Send materials to collaborators

West Coast Green Crab Experiment:

• Reorganize -80ºC so I can find all my samples
• Update summer notebook
• Identify samples for metabolomics and transcriptomics sequencing

Cold Acclimation Green Crab Experiment:

• Return to troubleshooting DNA extractions
• Return to troubleshooting triglyceride assay

Coral Transcriptomics:

These are things I need to do and can probably knock out in a week, but I think they’re a lower priority right now for me since there’s more momentum on other projects. At the very least, I need to reach out to collaborators and get this going again.

• Revise abstract
• Write discussion
• Write introduction

Other:

• Present invited seminars at Bigelow and Colby College
• Apply for relevant jobs

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Hawaii Gigas Methylation:

I’ll return to this when the CEABiGR paper is published.

• Finish methylKit contingency tests
• Conduct a randomization test for DML identification
• Integrate other epigenetic datasets
• Update methods and results
• Revise discussion