It’s time to extract more protein.

Based on the samples Alex used for lipid analysis, I’ve picked the following samples for my next round of extractions. I will extract 10 more samples from round 1, and 20 samples from round 2. This way, I can understand how site location, eelgrass condition, and outplant duration affected protein expression. These are just initial thoughts, and will likely change due to sample size considerations.

Here are the samples I’ve already analyzed:

Sample Site	Bare	Eelgrass
Case Inlet (CI)	O15	O07
Fidalgo Bay (FB)	O47	O37
Port Gamble Bay (PG)	O55	O77
Skokomish River Delta (SK)	O119	O107
Willapa Bay	O127	O142

Here are the samples I will analyze for my next round of extractions from the first outplant:

Sample Site	Bare	Eelgrass
Case Inlet (CI)	O11	O01
Fidalgo Bay (FB)	O64	O70
Port Gamble Bay (PG)	O25	O71
Skokomish River Delta (SK)	O96	O91
Willapa Bay	O135	O131

And samples for the second outplant. One thing I noticed was that there was only one eelgrass sample collected for Case Inlet:

Sample Site	Bare	Eelgrass
Case Inlet (CI)	O218	O222
Case Inlet (CI)	O217	N/A
Case Inlet (CI)	O219	N/A
Fidalgo Bay (FB)	O267	O209
Fidalgo Bay (FB)	O257	O214
Port Gamble Bay (PG)	O297	O280
Port Gamble Bay (PG)	O281	O292
Skokomish River Delta (SK)	O182	O184
Skokomish River Delta (SK)	O197	O202
Willapa Bay	O151	O165
Willapa Bay	O161	O174