SRM Analysis Part 5

Everything sucks.

R script

I normalized my data using TIC values, saved in my sequence file. After dividing all of my area values by TIC content, I remade my NMDS plot. Things did not look much better :disappointed:

tech-rep-cluster

During my meeting with Julian, he suggested I calculate the distances between ordinations for each technical replicate so I have a quantitative way of pulling out bad technical replicate clustering. For my bad NMDS, the distances didn’t look great :cold_sweat:

tech-rep-distances

Emma said she’s never seen such bad technical replication. Will I have yet another failed experiment? Will I be able to present anything at PCSGA? Who could never be sure :sob:

Written on September 8, 2017