More borrowed PRTC = All samples prepared

The assay is still going well! Emma brought down some more QC and PRTC for me to use. I added 30 µL of the final acetonitrile solvent to the 10 µL of PRTC + BSA, then added that to the mass spetrometer vial. I will need to replace the QC after about twelve more QC injections. I also added 30 µL more acetonitrile solution into the blank vial. I then diluted the 20 µL of stock PRTC from Emma with 30 µL of the final acetonitrile solvent to make 50 µL of a 0.2 pmol/µL PRTC solution, following this protocol. I then prepared the remainder of my samples and placed them on the second plate of the mass spectrometer. I had to remake O66 because when I tried pipetting 15 µL into the sample vial, there was not enough solution.

Table 1. Sample order on second plate for the first injection.

Plate 2	1	2	3	4	5
B	O32	O60	O101	O91	O100
C	O137	O96	O46	O90	O147
D	O30	O31	O131	O35	O24
E	O43	O40	O26	O78	O124
F	O64	O140	O66	O121	OBLNK2

I took the samples that were on Plate 1 and placed them in the freezer. I then added the order for the Plate 2 samples to the sequence file. Since I have to go to Manchester tomorrow, Emma agreed to help monitor the mass spectrometer. I sent her the plate arrangements for the second injection, and asked her to fun the samples in the same order they were on the plate.

Table 2. Sample order on first plate for the second injection.

Plate 1	1	2	3	4	5
B	O17	O56	O106	O10	O51
C	O145	O71	O49	O06	O21
D	O22	O12	O52	O122	O39
E	O128	O103	O14	O01	O118
F	O113	O04	O102	O99	O08

Table 3. Sample order on second plate for the second injection.

Plate 2	1	2	3	4	5
B	O137	O30	O40	O32	O96
C	O46	O100	O64	O147	O90
D	O43	O78	O91	O26	O131
E	O31	O35	O140	O121	O60
F	O66	O101	O124	O24	OBLNK2