I got a good amount of work done last month, but not as much as I needed to! However, I’m still feeling motivated so I’m going to ride that motivation before and after my end-of-summer vacation.

August Goals Recap

August Goals

Green Crab Pilot Experiment:

• Finalized metabolomics analysis!
• Created metabolomics figures!
• Updated methods and results

West Coast Green Crab Experiment:

…still didn’t do anything with this

Cold Acclimation Green Crab Experiment:

• Analyzed WA TTR data!

Killifish Methylation and Expression:

• Examined DMR-DEG overlaps!
• Ran BAT_correlating!
• Created DMR figures!
• Updated methods and results

Other:

• Supervised Catlin and Heidi as they analyze data and make their final presentations!
• Submitted SICB abstract for short-term acclimation paper
• Updated CV
• Submitted CEABiGR paper revision!
• Drafted NSF ORCC project summary

September Goals

Green Crab Pilot Experiment:

• Repeated measures analysis without crab ID for TTR data
• Finalize lipidomics analysis
• Determine if physiology data can be integrated with -omics data
• Determine if metabolomics and lipidomics data can be integrated
• Update methods
• Update results
• Write discussion
• Write introduction
• Write abstract

West Coast Green Crab Experiment:

• Update summer notebook
• Identify samples for metabolomics and lipidomics sequencing

Cold Acclimation Green Crab Experiment:

• Return to troubleshooting DNA extractions
• Return to troubleshooting triglyceride assay

NSF ORCC:

• Reread relevant literature
• Finalize project summary
• Full grant draft
• Send materials to collaborators

Job Applications:

• Create job application schedule
• Update job application materials
• Apply for relevant jobs

Killifish Methylation and Expression:

• Update methods and results
• Assist with discussion writing

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Coral Transcriptomics:

These are things I need to do and can probably knock out in a week, but I think they’re a lower priority right now for me since there’s more momentum on other projects.

• Revise abstract
• Write discussion
• Write introduction

Hawaii Gigas Methylation:

I’ll return to this when the CEABiGR paper is published.

• Finish methylKit contingency tests
• Conduct a randomization test for DML identification
• Integrate other epigenetic datasets
• Update methods and results
• Revise discussion