By starting new things, maybe that means actually setting reasonable goals. Jury’s out on whether or not it’ll work.

July Goals recap

Green Crab Pilot Experiment:

• I finished the respirometry analysis! Kinda. It’s 80% done without removing the background because Julia wanted to use my script to analyze her data

West Coast Green Crab Experiment:

• Can confirm I am physically alive but maybe not all there mentally

Summer Students:

• Assisted with Lauren’s abstract for SACNAS
• Didn’t need to write Julia a letter after all, but I wrote one for Lauren’s SACNAS application!
• Assisted students with their independent projects and analysis
• Facilitated introductions between students and other WHOI scientists

Coral Transcriptomics:

• Started reworking metaanalysis

Hawaii Gigas Methylation:

• Added Rajan’s edits as comments to the draft manuscript

August Goals

Green Crab Pilot Experiment:

• Remove background respiration from oxygen consumption calculations
• Update methods and results

West Coast Green Crab Experiment:

• Shut down the 15ºC and 25ºC tanks during week 6
• Shut down the 5ºC tanks during week 7
• Investigate any discrepancies in crab labelling and update relevant spreadsheets

Hawaii Gigas Methylation:

• Get other epigenetic datasets
• Finish methylKit contingency tests
• Conduct a randomization test for DML identification
• Integrate with other epigenetic datasets
• Update methods and results

Other:

• Review paper for Evolutionary Applications
• Review paper for Aquaculture
• Start draft of Evolution Symposium
• Revise CV
• Revise research statement
• Revise teaching statement

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Virginica Methylation and Expression:

I’ll return to this when I have an updated discussion for the Hawaii paper.

• Update methods and results from quadratic addition to transcriptional noise model
• Compare transcriptional noise analysis with Liew and Aranda papers

Killifish Methylation and Expression:

I’ll return to this when I have a good draft of the coral paper.

• Pathway analysis for RNA-Seq data
• Determine protocol for SNP identification
• Conduct in silico RRBS digestion
• Characterize DEG that overlap between populations
• Quantify gene expression variability
• Visualize DEG data

Zebrafish DNMT:

I’ll think about this once I have a robust killifish paper draft.

• Identify a stress condition to use for the zebrafish based on disease factors or conditions that are known to induce changes in methylation
• Determine physiological endpoints to assess for embryos
• Construct a project timeline