What last-minute panic is better than end-of-the-year panic?! I’ve got 17 days of time in Seattle before heading back to California for a bit of a break, so let’s see if my work expectations will actually meet my reality.

November Goals Recap

Virginica Labwork:

• Started checking DNA and RNA quality, but found out shortly after I started the process that I didn’t need to do that!
• Re-extracted DNA (and RNA) from two low-yield female samples
• Identified a protocol for sperm samples but did not test it

Coral Methylation Comparison:

• Calculated genome-wide methylation, but didn’t finalize contingency tests or the manuscript

Other:

• Submitted the NSF PRFB!
• Finalized ocean acidification and reproduction reivew for friendly reviewers!

December Goals

Virginica Labwork:

• Extract sperm DNA and RNA
• Send nucleic acids for library preparation
• Determine if there are mantle samples I want to extract

ATAC-Seq Labwork:

• Purchase reagents and identify samples to test cell dissociation protocols
• Ensure protocol is easy to follow and is accessible for the lab
• Dissociate and cryopreserve some cells

Gigas Labwork:

• If there’s some lag time while waiting for ATAC-Seq reagents, test RNA extraction protocol
• Extract RNA from samples sent for WGBS
• Send RNA for library preparation and sequencing
• Identify location of larval samples for potential extractions

Coral Methylation Comparison:

• Perform contingency tests for genomic location
• Update methods and results
• Contribute to the discussion
• Finalize manuscript for NOAA internal review!