November 2020 Goals

October Goals Recap

Virginica Labwork:

Gigas Gonad Labwork:

  • Ordered PAXgene kit for RNA extractions

ATAC-Seq Labwork:

  • I’m postponing thinking about ATAC-Seq preparations until I have a better idea of what C. virginica and C. gigas samples I have left after DNA/RNA extractions

Coral Methylation Comparison:

  • We restructured and streamlined the manuscript, but I have yet to address the specific edits and comments in my section

Other:

  • Finalized figures for the ocean acidification and reproduction review
  • Didn’t get a chance to create WGCNA and GO-MWU figures for Z. marina analyses
  • Coached two students through the NSF GRFP application process! Their final products were astounding.
  • Submitted 2 postdoc applications

November Goals

Virginica Labwork:

  • Test Zymo Microprep kit with sperm samples
  • Extract sperm DNA and RNA
  • Check DNA and RNA quality
  • Send nucleic acids for library preparation
  • Determine if there are mantle samples I want to extract

Gigas Labwork:

  • Test RNA extraction protocol
  • Extract RNA from samples sent for WGBS
  • Send RNA for library preparation and sequencing

ATAC-Seq Labwork:

  • If all other extractions are complete, purchase reagents and identify samples to test cell dissociation protocols

Coral Methylation Comparison:

  • Finalize manuscript for submission!

Other:

  • Submit the NSF PRFB
  • Finalize ocean acidification and reproduction reivew for friendly reviewers
  • Jump back into the Z. marina project
  • Survive
Written on November 1, 2020