As the new year starts I’m entering the interpretation and writing state for a lot of my projects, so this orange cat speaks to me.

December Goals recap

Green Crab Pilot Experiment:

• Conducted multivariate metabolomics analysis and functional enrichment!
• Did the same thing with the lipidomics data!
• Drafted talk for SICB

Coral Transcriptomics:

• Finished interpreting gene lists! I (affectionately, I think) called it my paper lockdown because I spent the majority of December working on this project.
• Outlined discussion

Hawaii Gigas Methylation:

• one day…I’ll actually work on this and meet my goals

West Coast Green Crab Experiment:

• Revisited Julia’s samples for genotyping
• Decided not to return to decontamination station since I’d be gone from the lab again, and would likely have to deal with a whole new set of environments

Other:

• Reviewed paper for PLOS Climate
• Reviewed paper for Biological Bulletin
• Wrote and submitted an AGA Special Event grant for RIE2

January Goals

West Coast Green Crab Experiment:

• Return to decontamination station and get going again on extractions
• Train Vanessa in extraction and genotyping workflow

Coral Transcriptomics:

• Edit methods and results
• Revise discussion outline
• Write discussion
• Write abstract
• Outline introduction
• Write introduction

Green Crab Pilot Experiment:

• Update methods and results from metabolomics and lipidomics analyses
• Finalize metabolomics analysis
• Finalize lipidomics analysis
• Review green crab thermal physiology literature

Hawaii Gigas Methylation:

• Finish methylKit contingency tests
• Conduct a randomization test for DML identification
• Integrate other epigenetic datasets
• Update methods and results
• Revise discussion

Other:

• Submit additional faculty applications
• Review NSF proposal
• Launch RIE2 survey
• Work on PRISMA searches for WOCinEEB Global Change collaboration

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Virginica Methylation and Expression:

I’ll return to this when I have an updated discussion for the Hawaii paper.

• Update methods and results from quadratic addition to transcriptional noise model
• Compare transcriptional noise analysis with Liew and Aranda papers

Killifish Methylation and Expression:

I’ll return to this when I have a good draft of the coral paper.

• Pathway analysis for RNA-Seq data
• Determine protocol for SNP identification
• Conduct in silico RRBS digestion
• Characterize DEG that overlap between populations
• Quantify gene expression variability
• Visualize DEG data

Zebrafish DNMT:

I’ll think about this once I have a robust killifish paper draft.

• Identify a stress condition to use for the zebrafish based on disease factors or conditions that are known to induce changes in methylation
• Determine physiological endpoints to assess for embryos
• Construct a project timeline