Reproductive Output Analyses
i.e. My grand return to RStudio
It’s been a while since I did any stats-related! I tackled the preliminary analysis of the egg production and hatch rate data from Manchester. Since my original data was in an .xlsx file with several different tabs, I converted the relevant tabs into separate .csv files. I then created a new R script and started coding some ANOVAs.
There were significant differences in egg production between treatments (F = 25.87017, p = 0.00112206). Females exposed to heat shock produced more eggs than females exposed to either low or ambient pH treatments (HS-A = 0.0022743; L-HS = 0.0016528).
There was only a significant difference between treatments when taking female treatment into account (F = 5.606537, p = 0.01039109). This was due to a difference between low and ambient pH hatch rates, with low pH hatch rates being less than ambient pH hatch rates (L-A = 0.0111872). This is what I suspected when I first looked at this data!
Females exposure to low pH treatments could explain lower larval hatch rates #MaternalEffect Since there was no difference in the number of eggs produced between females exposed to low and ambient pH treatments, perhaps low pH eggs are lower quality. There could also be an epigenetic effect at play (cue MBD-seq preparation…or at least cue bringing this finding to Steven’s attention and hoping there’s money in the budget).
Once I sort out my histology data, I think I’ll have a story to tell for NSA.