I truly feel like the past month has been the epitome of me running around like a headless chicken. This month is going to be a lot of them same since I’m going on vacation for a bit over two weeks!

April Goals recap

Green Crab Summer Experiment:

• Created an experimental plan and timeline
• Met with the two SSF and PEP students to plan summer projects! They both seem awesome and I’m excited to work with them on independent projects
• Coordinated logistics for summer experiments with MFW, WDFW, and folks at ESL
• Budgeted for experimental materials using PDS and NSF PRFB funds

Green Crab Pilot Experiment:

• Updated experimental methods
• I didn’t finish demographic data analysis or get started on the respirometry analysis, but I did finish the survivorship and time-to-right analyses!
• I budgeted for metabolomics materials and contacted the sequencing facility, but they are booked out and said they would contact me in 3 months. Based on their availability, I may look into other metabolomics cores and determine the cost-effectiveness of sending samples to be prepped externally versus in-house

Coral Transcriptomics:

• Since Ann, Maggie, and I all had various travel obligations, I took a break from this project in April, knowing that we’d reconvene later.

Virginica Methylation and Expression:

• Updated results with initial mixOmics analysis
• Discussed the modeling efforts further to include a quadratic relationship between gene expression and transcriptional noise

Hawaii Gigas Methylation:

• Didn’t do anything for this project :/

Other:

• Also didn’t look into the mindful productivity course. Whoops.

May Goals

Green Crab Summer Experiment:

• Determine a reasonable biochemistry project for Lauren
• Order experimental materials

Green Crab Pilot Experiment:

• Demographic data analysis
• Analyze respirometry data
• Update methods and results

Hawaii Gigas Methylation:

• Add Rajan’s edits as comments to the draft manuscript
• Review EpiDiverse output and create a new SNP list
• Rerun methylKit
• Conduct a randomization test for DML identification
• Update methods and results

Virginica Methylation and Expression:

• Update methods and results from quadratic addition to transcriptional noise model
• Compare transcriptional noise analysis with Liew and Aranda papers

Other:

• MINDFUL PRODUCTIVITY MODULE I

Deep Freeze

Things that are not immediate enough to be on the backburner, so they’re stuck in the deep freeze.

Killifish Methylation and Expression:

I’ll return to this when I have a good draft of the coral paper.

• Pathway analysis for RNA-Seq data
• Determine protocol for SNP identification
• Conduct in silico RRBS digestion
• Characterize DEG that overlap between populations
• Quantify gene expression variability
• Visualize DEG data

Zebrafish DNMT:

I’ll think about this once I have a robust killifish paper draft.

• Identify a stress condition to use for the zebrafish based on disease factors or conditions that are known to induce changes in methylation
• Determine physiological endpoints to assess for embryos
• Construct a project timeline